BCIP 5-溴-4-氯-3-吲哚基磷酸酯对甲苯胺盐

CAS 6578-06-9 MFCD00040636

化学结构图

6578-06-9
SMILES: Cc1ccc(N)cc1.O=P(O)(O)Oc1c[nH]c2ccc(Br)c(Cl)c12

化学属性

Mol. FormulaC8H6BrClNO4P·C7H9N
Mol. Weight434
Density1.422
Melting Point196-198°C
Appearance tablets
SolubilityDMF: 20 mg/mL
Stability对光线敏感,对空气敏感,对热敏感

别名和识别编码

Chemical NameBCIP
Synonym 5-溴-4-氯-3-吲哚基磷酸酯对甲苯胺盐 5-溴-4-氯-3-吲哚磷酸 对甲苯胺盐 BCIP BCIP P-TOLUIDINE SALT X-phosphate p-toluidine salt
CAS Number6578-06-9
PubChem Substance ID87564175
EC Number229-506-1
MDL NumberMFCD00040636
Reaxys-RN15195285
Beilstein Registry Number2384789
Chemical Name Translation5-溴-4-氯-3-吲哚基磷酸酯对甲苯胺盐
InChIKeyQEIFSLUFHRCVQL-UHFFFAOYSA-N
LabNetwork Molecule IDLN01254908
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分类

  • {SNA} Alkaline Phosphatase Substrates for Western Blotting, Alphabetical Listing, BCIP/NBT Substrate Systems, Colorimetric, Core Bioreagents, Detection, Detection Substrates, Enzyme Substrates, Life Science Reagents for Western Blotting, Research Essentials, Substrates, Tablets,

产品应用

  • 碱性磷酸酶(alkaline phosphatase)的底物。
  • 5-溴-4-氯-3-吲哚磷酸(BCIP) 和 氯化硝基四氮唑蓝(NBT)用于比色法鉴定碱性磷酸酶标记分子. BCIP/NBT 底物系统用途非常广泛:包括 Northern Southern, Western blotting, 原位杂交(in situ hybridization)和免疫组织化学。
  • BCIP 可以有两种盐的形式: 二钠盐型溶于水,对甲苯胺型溶于二甲基甲酰胺(DMF)。这些盐型可用于制备和NBT结合的储存溶液和反应缓冲液,构成碱性磷酸酶的底物溶液。 底物系统和碱性磷酸酶一起培养,产生一种不溶的带紫色的NBT双甲腊( diformazan )产物,容易被观察。

相关文献及参考

  • McGadey, J., A tetrazolium method for non-specific alkaline phosphatase. Histochemie 23 , 180-184, (1970) 摘要
  • Horwitz, J.P., et al. J. Med. Chem. 9 , 447, (1966)
  • Leary, J.J., et al., Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots. Proc. Natl. Acad. Sci. U. S. A. 80 , 4045-4049, (1983)
  • Knecht, D.A., Dimond, R.L., Visualization of antigenic proteins on Western blots. Anal. Biochem. 136 , 180, (1984)
  • Walters, C., et al., Detection of parvovirus B19 in macerated fetal tissue using in situ hybridization. J. Clin. Pathol. 50(9) , 749-754, (1997) 摘要
  • Meltzer, J.C., et al., Enhanced immunohistochemical detection of autonomic nerve fibers, cytokines and inducible nitric oxide synthase by light and fluorescent microscopy in rat spleen. J. Histochem. Cytochem. 45(4) , 599-610, (1997) 摘要

安全信息

GHS Symbol
WGK Germany3
Hazard Codes Xi
Hazard statements
  • H315 Causes skin irritation 会刺激皮肤
  • H319 Causes serious eye irritation 严重刺激眼睛
  • H335 May cause respiratory irritation 可能导致呼吸道刺激
  • H301+H311+H331
Personal Protective Equipment dust mask type N95 (US), Eyeshields, Gloves
Precautionary statements
  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray. 避免吸入粉尘/烟/气体/烟雾/蒸汽/喷雾。
  • P264 Wash hands thoroughly after handling. 处理后要彻底洗净双手。
  • P270 Do not eat, drink or smoke when using this product. 使用本产品时不要吃东西,喝水或吸烟。
  • P271 Use only outdoors or in a well-ventilated area.? 只能在室外或通风良好的地方使用。
  • P280 Wear protective gloves/protective clothing/eye protection/face protection. 戴防护手套/防护服/眼睛的保护物/面部保护物。
  • P301+P310+P330
  • P302+P352+P312+P361+P364
  • P304+P340+P311
  • P305+P351+P338
  • P403+P233
  • P405 Store locked up. 上锁保管。
  • P501 Dispose of contents/container to..… 处理内容物/容器.....
Signal word Warning
Storage condition <;0 <0°C 储存温度: -20°C 2-8°C 储存温度: -20°C,充氩保存
UN Number 2811
Safety Statements
  • S26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice 眼睛接触后,立即用大量水冲洗并征求医生意见;
Risk Statements
  • R36/37/38 Irritating to eyes, respiratory system and skin 对眼睛、呼吸系统和皮肤有刺激性
Packing GroupIII
Hazard Class6.1

其他信息

  • 5-溴-4-氯-3-吲哚基磷酸酯对甲苯胺盐价格(试剂级):更新日期 产品编号 产品名称 包装 价格 2011/08/23 xw020009 5-溴-4-氯-3-吲哚磷酸酯对甲苯胺盐 (BCIP)(-20℃) 250mg 650元 2011/04/16 B1239 5-溴-4-氯-3-吲哚基磷酸酯对甲苯胺盐[用于生化研究] 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt [for Biochemical Research] 1G 3120元 2011/04/16 B1239 5-溴-4-氯-3-吲哚基磷酸酯对甲苯胺盐[用于生化研究] 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt [for Biochemical Research] 100MG 592元
  • For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indoly?l phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
  • MOL 文件:6578-06-9.mol
  • F:3-8-10
  • Sigma Aldrich:6578-06-9(sigmaaldrich)
  • TCI Shanghai:5-溴-4-氯-3-吲哚基磷酸酯对甲苯胺盐[用于生化研究] 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt [for Biochemical Research],>;98.0%(T)(6578-06-9)
  • 用途一:碱性磷酸酶(alkaline phosphatase)的组织化学底物。5-溴-4-氯-3-吲哚磷酸(BCIP) 和 氯化硝基四氮唑蓝(NBT)用于比色法鉴定碱性磷酸酶标记分子. BCIP/NBT 底物系统用途非常广泛:包括 Northern Southern, Western blotting, 原位杂交(in situ hybridization)和免疫组织化学。BCIP 可以有两种盐的形式: 二钠盐型溶于水,对甲苯胺型溶于二甲基甲酰胺(DMF)。这些盐型可用于制备和NBT结合的储存溶液和反应缓冲液,构成碱性磷酸酶的底物溶液。 底物系统和碱性磷酸酶一起培养,产生一种不溶的带紫色的NBT双甲腊( diformazan )产物,容易被观察。

系列性分类


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