• 原料直供
• 试剂商城
• 直接询价

分类

• {SNA} Amino Acid Analysis,
• {SNA} Building Blocks, C13 to C42+, Carbonyl Compounds, Carboxylic Acids, Chemical Synthesis, Organic Building Blocks
• {SNA} Building Blocks, C13 to C42+, Carbonyl Compounds,
• {SNA} Amino Acid Analysis, Amino Acid Analysis Reagents, Analytical and Industrial Enzymes, Chemical Synthesis, Cholinesterase and Organophosphate Detection Reagents, Detection Reagents,
• {SNA} Amino Acid Analysis, Amino Acid Analysis Reagents, Analytical and Industrial Enzymes, Biochemicals and Reagents, Cholinesterase and Organophosphate Detection Reagents, Detection Reagents, Enzymes, Inhibitors, and Substrates, Peptide Analysis and Characterization, Peptides
• {SNA} Amino Acid An

产品应用

• 巯基测定，是测定蛋白质、多肽和组织中巯基的灵敏试剂。也用于有机磷农药中毒的监测（胆碱酯酶测定）。

相关文献及参考

• Reacts with free thiol groups to form a highly colored (yellow) thiolate anion: Arch. Biochem. Biophys., 82, 70 (1959); used for quantitative determination of thiols. Also used in chromogenic assay of acetylcholinesterase: Enzyme Assays, R. Eisenthal and M. J. Danson, Eds., OUP, Oxford (1992), p81.

安全信息

GHS Symbol

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intraperitoneal
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 2080 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   ARZNAD Arzneimittel-Forschung. Drug Research.  (Editio Cantor Verlag,
   Postfach 1255, W-7960 Aulendorf, Fed. Rep. Ger.)  V.1-    1951-
   Volume(issue)/page/year: 21,284,1971

其他信息

• Highlights:
• Highlights: ?Sulfhydryl assay – reacts quantitatively with free (reduced) sulfhydryl groups (–SH) to yield detectable TNB product ?Colorimetric detection – colored product enables spectrophotometric measurement in cuvettes or microplates (λmax = 412nm; ε = 14,150/M?cm) ?Proven method – well-characterized chemistry provides quantitation of peptide or protein sulfhydryl groups using extinction coefficient calculation or by comparison to a cysteine standard
• Alfa Aesar:5,5'-二硫双(2-硝基苯甲酸),99% 5,5'-Dithiobis(2-nitrobenzoic acid), 99%(69-78-3)
• {Chemi
• TCI Shanghai:5,5'-二硫代双(2-硝基苯甲酸)[用于巯基基团的测定] 5,5'-Dithiobis(2-nitrobenzoic Acid) [for Determination of SH groups],>;98.0%(T)(69-78-3)
• 用途一:广泛用于生化研究，是生化反应色原，诊断试剂研究生产，测定蛋白质、多肽和组织中巯基的灵敏测定试剂。有机磷农药中毒的监测（胆碱酯酶测定）。
• 海关编码:29309070
• Ellman's Reagent (5,5'-dithio-bis-[2-nitrobenzoic acid]) is used to estimate sulfhydryl groups in a sample by comparing to a standard curve of a sulfhydryl-containing compound such as cysteine. Alternatively, sulfhydryl groups can be assayed using the extinction coefficient of TNB (14,150M-1cm-1 at 412nm). Ellman's Reagent has been used for applications other than sulfhydryl estimation, such as for determining alkylthiols by HPLC using a pre-column derivatization procedure and to study thiols in the active site of several enzymes.
• MOL 文件:69-78-3.mol
• Ellman's Reagent (DTNB) reacts with sulfhydryl groups to yield a colored product, providing a reliable method to measure reduced cysteines and other free sulfhydryls in solution.
• 5,5'-二硫双(2-硝基苯甲酸)价格(试剂级):更新日期 产品编号 产品名称 包装 价格 2011/08/23 30078984 5，5’-二硫代双（2-硝基苯甲酸） 5g 650元 2011/08/23 L0986802 5.5’-二硫代双（2-硝基苯甲酸）99％ 5g 607元 2011/04/16 D0944 5,5'-二硫代双(2-硝基苯甲酸)[用于巯基基团的测定] 5,5'-Dithiobis(2-nitrobenzoic Acid) [for Determination of SH groups] 5G 776元
• MSDS 信息:3-Carboxy-4-nitrophenyl disulfide(69-78-3).msds
• F:10