Acid Black 1 酸性黑1

CAS 1064-48-8 MFCD00004017

化学结构图

1064-48-8
SMILES: Nc1c(/N=N/c2ccc([N+](=O)[O-])cc2)c(S(=O)(=O)[O-])cc2cc(S(=O)(=O)[O-])c(/N=N/c3ccccc3)c(O)c12.[Na+].[Na+]

化学属性

Mol. FormulaC22H14N6Na2O9S2
Mol. Weight616.49
TSCAYes
Density1.05
Solubility10 G/L (25 ºC)
Stability易吸潮
Flash Point30°C
Melting Point>350 °C

别名和识别编码

Chemical NameAcid Black 1
Synonym Amido Black 10B {LY} Amido Black 10B {} {LY} Amido Black 10B {} {} {LY} Amido Black 10B {} {} {} {LY} Amido Black 10B {} {} {} {} {LY} Amido Black 10B {} {} {} {} {} {LY} Amido Black 10B {} {} {} {} {} {} {LY} Amido Black 10B {} {} {} {} {} {} {} {LY} Amido Black 10B {} {} {} {} {} {} {} {} {LY} Amido Black 10B {} {} {} {} {} {} {} {} {} {LY} Amido Black 10B {} {} {} {} {} {} {} {} {} {} { {} {} {} {} {} {} {} {} {} {} {LY} Amido Black 10B {} {} {} {} {} {} {} {} {} {} {} {LY} Amido Black 10B {} {} {} {} {} {} {} {} {} {} {} {} {LY} Amido Black 10B {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Amido Black 10B {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Amido Black 10B {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} { {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Amido Black 10B {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} { {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Amido Black 10B {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Amido
CAS Number1064-48-8
PubChem Substance ID87560295
EC Number213-903-1
Beilstein Registry Number5374263
MDL NumberMFCD00004017
Reaxys-RN739194
Chemical Name Translation酸性黑1
LabNetwork Molecule IDLN00223183
InChIInChI=1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2
Canonical SMILESO=S(C1=C(/N=N/C2=CC=C([N+]([O-])=O)C=C2)C(N)=C3C(O)=C(/N=N/C4=CC=CC=C4)C(S(=O)(O[Na])=O)=CC3=C1)(O[Na])=O
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分类

  • {SNA} Other Stains for Electrophoresis, Protein Stains and Staining Reagents, 分子生物学, 蛋白电泳, 蛋白质组学
  • {SNA} Buffers, Reagents, & Stains,
  • {SA} Protein Sequenci
  • Buffers, Reagents, & Stains, Core Bioreagents, Life Scienc
  • {Chemicalbook} 染料: 酸性染料: 强酸性染料
  • {SNA} Molecular Biology, Other Stains for Electrophoresis, Protein Electrophoresis, Protein Stains and Staining Reagents,
  • {SNA} N,

产品应用

  • 用于对聚丙烯酰胺凝胶、琼脂糖凝胶和硝酸纤维素膜上蛋白质的染色。在电泳后，推荐固定蛋白质在胶上。 胶用 0.1% 氨基黑10B的7% (v/v) 醋酸溶液染至少2 小时，然后用7% (v/v) 醋酸溶液脱色。检测灵敏度大概是考马斯亮蓝R250的20%.

相关文献及参考

  • Beilstein: 16,IV,622
  • 2. Wilson, C.M., Staining of proteins on gels: Comparisons of dyes and procedures. Meth. Enzymol. 91 , 236-247, (1983) 摘要

安全信息

GHS Symbol
WGK Germany3
Hazard Codes Xi,C,F Xi
Hazard statements
  • H226 Flammable liquid and vapour 易燃液体和蒸气
  • H315 Causes skin irritation 会刺激皮肤
  • H319 Causes serious eye irritation 严重刺激眼睛
  • H335 May cause respiratory irritation 可能导致呼吸道刺激
  • H336 May cause drowsiness or dizziness 肯能导致嗜睡或头晕
Personal Protective Equipment dust mask type N95 (US), Eyeshields, Gloves
RTECSQJ6196000
Precautionary statements
  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray. 避免吸入粉尘/烟/气体/烟雾/蒸汽/喷雾。
  • P264 Wash hands thoroughly after handling. 处理后要彻底洗净双手。
  • P280 Wear protective gloves/protective clothing/eye protection/face protection. 戴防护手套/防护服/眼睛的保护物/面部保护物。
  • P302+P352+P332+P313+P362+P364
  • P305+P351+P338
  • P305+P351+P338+P337+P313
  • P362 Take off contaminated clothing and wash before reuse. 脱掉污染的衣服，清洗后方可重新使用
Signal word Warning
Safety Statements
  • S24/25 Avoid contact with skin and eyes 避免皮肤和眼睛接触；
  • S26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice 眼睛接触后，立即用大量水冲洗并征求医生意见；
Risk Statements
  • R36/37/38 Irritating to eyes, respiratory system and skin 对眼睛、呼吸系统和皮肤有刺激性
Storage condition Store at room temperature {LY} Store at room temperature {} {LY} Store at room temperature {} {} {LY} Store at room temperature {} {} {} {LY} Store at room temperature {} {} {} {} {LY} Store at room temperature {} {} {} {} {} {LY} Store at room temperature {} {} {} {} {} {} {LY} Store at room temperature {} {} {} {} {} {} {} {LY} Store at room temperature {} {} {} {} {} {} {} {} {LY} Store at room temperature {} {} {} {} {} {} {} {} {} {LY} Store at room temperature {} {} {} {} {} {} {} {} {} {} {LY} Store at room temperature {} {} {} {} {} {} {} {} {} {} {} {LY} Store at room temperature {} {} {} {} {} {} {} {} {} {} {} {} {LY} Store at room temperature {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Store at room temperature {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Store at room temperature {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Store at room temperature {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}
UN Number UN 2924 3/PG 3 
TYPE OF TEST            : Mutation in microorganisms
TEST SYSTEM             : Bacteria - Salmonella typhimurium
DOSE/DURATION           : 50 ug/plate
REFERENCE :
   EMMUEG Environmental and Molecular Mutagenesis.  (Alan R. Liss, Inc., 41 E.
   11th St., New York, NY 10003)  V.10-    1987-  Volume(issue)/page/year:
   22,188,1993

其他信息

  • 稳定性:Stable. Incompatible with strong oxidizing agents.
  • 生产方法:以 H 酸、对硝基苯胺、苯胺为原料，首先将对硝基苯胺重氮化，于酸性条件下与 H 酸偶合，然后将苯胺重氮化，于碱性条件下与前述偶合产物进行第二次偶合即得产物。经盐析、过滤、干燥、粉碎得成品。。
  • 海关编码:32041200
  • 色指数:20470
  • 图谱信息:酸性黑 1(1064-48-8)红外图谱(IR1) 酸性黑 1(1064-48-8)红外图谱(IR2)
  • EPA化学物质信息:2,7-Naphthalenedisulfonic acid, 4-amino-5-hydroxy-3-[(4-nitrophenyl) azo]-6-(phenylazo)-, disodium salt(1064-48-8)
  • 酸性黑 1价格(试剂级):更新日期 产品编号 产品名称 包装 价格 2011/08/23 71003243 氨基黑10B;萘酚蓝黑;水牛黑NBR;酸性黑1;4-氨基-5-羟基-3-(对硝基苯偶氮)-6-(苯偶氮)-2,7-萘二磺酸二钠盐 10g 14元 2011/08/23 71003380 氨基黑10B;萘酚蓝黑;水牛黑NBR;酸性黑1;4-氨基-5-羟基-3-(对硝基苯偶氮)-6-(苯偶氮)-2,7-萘二磺酸二钠盐 25g 23.9元 2011/04/16 A0586 酸性黑1 Acid Black 1 25G 309元
  • RTECS号:QJ6196000
  • 可溶于水，呈蓝黑色。溶于酒精呈蓝色，微溶于丙酮，不溶于其他有机溶剂。遇浓硫酸呈蓝光绿色，将其稀释后生成暗绿色光的蓝色沉淀；遇浓硝酸呈暗绿色溶液，后转变为酱红色；遇10%氢氧化钠溶液呈艳蓝色。其水溶液加浓盐酸生成绿光蓝色沉淀，加氢氧化钠液生成蓝色沉淀。染色时，遇铜、铁离子，其色泽前者微有变化，后者略带浅绿.
  • MOL 文件:1064-48-8.mol
  • 用途:主要用于羊毛、蚕丝、锦纶及其混纺织物的染色和印花。可与酸性橙Ⅱ拼制成酸性黑ATT，也可用于纸张、电化铝、肥皂、木材、生物、皮革、医药、化妆品的着色。还可用于制造墨水。

系列性分类

  1. Life Science 生命科学
  2. Hematology and Histology 血液学和组织学
  3. Stains and Dyes - Hematology and Histology
  4. Stains & Dyes, A to Z - Stains and Dyes
  5. N - Stains & Dyes, A to Z
  1. Life Science 生命科学
  2. Proteomics 蛋白质组学
  3. Protein Electrophoresis - Proteomics
  4. Protein Stains and Staining Reagents - Protein Electrophoresis
  5. Other Stains for Electrophoresis - Protein Stains and Staining Reagents
  1. Life Science 生命科学
  2. Proteomics 蛋白质组学
  3. Protein Structural Analysis - Proteomics
  4. Protein Sequencing - Protein Structural Analysis
  5. Reagents for Protein Sequencing - Protein Sequencing
  1. Functional Group 官能团索引
  2. azo compound

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