Safranine T 藏红T

CAS 477-73-6 MFCD00011759

化学结构图

477-73-6
SMILES: Cc1cc2nc3cc(C)c(N)cc3[n+](-c3ccccc3)c2cc1N.[Cl-]

化学属性

Mol. FormulaC20H19ClN4
Mol. Weight350.84
Density0.986 g/mL at 20 °C
Refractive indexn20/D 1.339
TSCAYes
SolubilitySoluble in water (50 mg/ml at 20 °C), and alcohol.
Stability对湿度敏感
Flash Point46 °C
Melting Point>240 °C
Boiling Point507.14 °C at 760 mmHg

别名和识别编码

Chemical NameSafranine T
Synonym Safranin {LY} Safranin {} {LY} Safranin {} {} {LY} Safranin {} {} {} {LY} Safranin {} {} {} {} {LY} Safranin {} {} {} {} {} {LY} Safranin {} {} {} {} {} {} {LY} Safranin {} {} {} {} {} {} {} {LY} Safranin {} {} {} {} {} {} {} {} {LY} Safranin {} {} {} {} {} {} {} {} {} {LY} Safranin {} {} {} {} {} {} {} {} {} {} {LY} Safranin {} {} {} {} {} {} {} {} {} {} {} {LY} Safranin {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Safranin {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Safranin {} {} {} {} {} {} {} {} {} {} {} {} {} {} { {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Safranin {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} { {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Safranin {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} { {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Safranin {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} { {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Safranin {} {} {} {} {} {} {
MDL NumberMFCD00011759
Beilstein Registry Number3924099
CAS Number477-73-6
EC Number207-518-8
Reaxys-RN3924099
PubChem Substance ID2723800
Chemical Name Translation藏红T
InChIKeyOARRHUQTFTUEOS-UHFFFAOYSA-N
LabNetwork Molecule IDLN00195469
InChIInChI=1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H
Canonical SMILESCC1=CC2=NC3=C([N+](C4=CC=CC=C4)=C2C=C1N)C=C(N)C(C)=C3.[Cl-]
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分类

  • {SNA} Analytical Reagents, Analytical/Chromatography, Indicators,
  • {SNA} Cell Biology, Certified Stains & Dyes, Core Bioreagents, Hematology and Histology, Research Essentials, S, Stains & Dyes, A to Z, Stains and Dy
  • {SNA} Analytical R
  • {SNA} Certified Stains & Dyes, Core Bioreagents, Research Essentials, S, Stains & Dyes, A to Z, Stains and Dyes, 细胞生物学, 血液学和组织学
  • {SNA} Microscopy Reagents and Kits, Staining Solutions, Staining Solutions for Gram Staining, 微生物学
  • Microbiology, Microscopy Reagents and Kits, Staining Solutions, Staining Solutions for Gram Staining
  • {Chemicalbook} 染料: 阳离子染料: 一般碱性染料
  • {SNA} Ana

产品应用

  • 微量分析亚硝酸的试剂。氧化还原指示剂、酸碱指示剂、生物染色剂。
  • 高纯级用在Flemming triple染色中的染色体染色；在吉姆萨染色中对革兰氏阴性细菌染色；在Safranine-fast green FCF染色中对植物细胞染色。

相关文献及参考

  • Biological stain.

安全信息

GHS Symbol
Hazard statements
  • H226 Flammable liquid and vapour 易燃液体和蒸气
  • H315 Causes skin irritation 会刺激皮肤
  • H318 Causes serious eye damage 严重伤害眼睛
  • H319 Causes serious eye irritation 严重刺激眼睛
WGK Germany3
Personal Protective Equipment Eyeshields, Faceshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter dust mask type N95 (US), Eyeshields, Gloves Eyeshields, Gloves
Signal word Danger Warning
RTECSSG1623000
Precautionary statements
  • P201 Obtain special instructions before use. 使用前获取专门指示。
  • P202 Do not handle until all safety precautions have been read and understood. 已阅读并理解所有的安全预防措施之前，切勿操作。
  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray. 避免吸入粉尘/烟/气体/烟雾/蒸汽/喷雾。
  • P264 Wash hands thoroughly after handling. 处理后要彻底洗净双手。
  • P280 Wear protective gloves/protective clothing/eye protection/face protection. 戴防护手套/防护服/眼睛的保护物/面部保护物。
  • P301+P312
  • P302+P352
  • P302+P352+P332+P313+P362+P364
  • P305+P351+
  • P305+P351+P338
  • P305+P351+P338+P337+P313
  • P308+P313
  • P310 Immediately call a POISON CENTER or doctor/physician. 立即呼救解毒中心或医生/医师。
  • P321 Specific treatment (see … on this label). 具体治疗（见本标签上的）。
  • P332+P313
  • P337+P313
  • P362 Take off contaminated clothing and wash before reuse. 脱掉污染的衣服，清洗后方可重新使用
  • P405 Store locked up. 上锁保管。
  • P501 Dispose of contents/container to..… 处理内容物/容器.....
Safety Statements
  • S24/25 Avoid contact with skin and eyes 避免皮肤和眼睛接触；
Risk Statements
  • R10 Flammable 易燃
  • R36/37/38 Irritating to eyes, respiratory system and skin 对眼睛、呼吸系统和皮肤有刺激性
  • R36/38 Irritating to eyes and skin 对眼睛和皮肤有刺激性
  • R37/38 Irritating to respiratory system and skin 对呼吸系统和皮肤有刺激性
  • R41 Risk of serious damage to eyes 有严重损伤眼睛的危险
Hazard Codes F Xi
Storage condition RT, sealed storage, away from moisture and light {LY} RT, sealed storage, away from moisture and light Store at RT. {} {LY} RT, sealed storage, away from moisture and light {} {} {LY} RT, sealed storage, away from moisture and light {} {} {} {LY} RT, sealed storage, away from moisture and light {} {} {} {} {LY} RT, sealed storage, away from moisture and light {} {} {} {} {} {LY} RT, sealed storage, away from moisture and light {} {} {} {} {} {} {LY} RT, sealed storage, away from moisture and light {} {} {} {} {} {} {} {LY} RT, sealed storage, away from moisture and light {} {} {} {} {} {} {} {} {LY} RT, sealed storage, away from moisture and light {} {} {} {} {} {} {} {} {} {LY} RT, sealed storage, away from moisture and light {} {} {} {} {} {} {} {} {} {} {LY} RT, sealed storage, away from moisture and light {} {} {} {} {} {} {} {} {} {} {} {LY} RT, sealed storage, away from moisture and light {} {} {} {} {} {} {} {} {} {} {} {} {LY} RT, sealed stor
UN Number UN 1170 3/PG 3 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - rabbit
DOSE/DURATION           : 26940 ug/kg
TOXIC EFFECTS :
   Behavioral - convulsions or effect on seizure threshold
   Lungs, Thorax, or Respiration - respiratory stimulation
   Lungs, Thorax, or Respiration - other changes
REFERENCE :
   SMBUA9 Stanford Medical Bulletin.  (Palo Alto, CA)  V.1-20, 1942-62.
   Discontinued.  Volume(issue)/page/year: 9,96,1951

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 28740 ug/kg
TOXIC EFFECTS :
   Behavioral - convulsions or effect on seizure threshold
   Lungs, Thorax, or Respiration - respiratory stimulation
   Lungs, Thorax, or Respiration - other changes
REFERENCE :
   SMBUA9 Stanford Medical Bulletin.  (Palo Alto, CA)  V.1-20, 1942-62.
   Discontinued.  Volume(issue)/page/year: 9,96,1951

TYPE OF TEST            : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE       : Oral
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 1600 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   JPMSAE Journal of Pharmaceutical Sciences.  (American Pharmaceutical Assoc.,
   2215 Constitution Ave., NW, Washington, DC 20037)  V.50-    1961-
   Volume(issue)/page/year: 69,327,1980

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 24020 ug/kg
TOXIC EFFECTS :
   Behavioral - convulsions or effect on seizure threshold
   Lungs, Thorax, or Respiration - respiratory stimulation
   Lungs, Thorax, or Respiration - other changes
REFERENCE :
   SMBUA9 Stanford Medical Bulletin.  (Palo Alto, CA)  V.1-20, 1942-62.
   Di

TYPE OF TEST            : DNA repair
TEST SYSTEM             : Bacteria - Bacillus subtilis
DOSE/DURATION           : 2 mg/disc
REFERENCE :
   TRENAF Kenkyu Nenpo--Tokyo-toritsu Eisei Kenkyusho.  Annual Report of Tokyo
   Metropolitan Research Laboratory of Public Health.  (Tokyo-toritsu Eisei
   Kenkyusho, 24-1, 3-chome, Hyakunin-cho, Shin-juku-ku, Tokyo 160, Japan) V.1-
      1949/50-  Volume(issue)/page/year: 27,153,1976

TYPE OF TEST            : Mutation in microorganisms
TEST SYSTEM             : Bacteria - Salmonella typhimurium
DOSE/DURATION           : 16 ug/plate
REFERENCE :
   TRENAF Kenkyu Nenpo--Tokyo-toritsu Eisei Kenkyusho.  Annual Report of Tokyo
   Metropolitan Research Laboratory of Public Health.  (Tokyo-toritsu Eisei
   Kenkyusho, 24-1, 3-chome, Hyakunin-cho, Shin-juku-ku, Tokyo 160, Japan) V.1-
      1949/50-  Volume(issue)/page/year: 27,153,1976

TYPE OF TEST            : DNA repair
TEST SYSTEM             : Bacteria - Escherichia coli
DOSE/DURATION           : 4 ug/well
REFERENCE :
   ENMUDM Environmental Mutagenesis.  (New York, NY)  V.1-9, 1979-87.  For
   publisher information, see EMMUEG.  Volume(issue)/page/year: 3,429,1981

其他信息

  • 稳定性:Stable.
  • 用途:用作微量分析亚硝酸的试剂、氧化还原指示剂、酸碱指示剂、生物染色剂
  • MOL 文件:477-73-6.mol
  • 色指数:50240
  • 图谱信息:碱性红 2(477-73-6)红外图谱(IR1) 碱性红 2(477-73-6)红外图谱(IR2)
  • 毒害物质数据:477-73-6(Hazardous Substances Data)
  • 碱性红 2价格(试剂级):更新日期 产品编号 产品名称 包装 价格 2011/08/23 71037844 藏红T;碱性藏红花;番红花红T;蓝光藏红花;盐基桃红;沙黄 25g 44.5元 2011/04/16 S0145 碱性红2 Basic Red 2 25G 383元 2011/04/16 S0145 碱性红2 Basic Red 2 500G 3640元
  • EPA化学物质信息:Phenazinium, 3,7-diamino-2,8-dimethyl-5- phenyl-, chloride(477-73-6)
  • 易溶于水成红色溶液，溶于乙醇成红色带黄红色荧光。对盐酸为蓝红色溶液，过多则呈紫色，大量过多则转为蓝色；对氢氧化钠则生成桲红色沉淀；硫酸则为绿色溶液，稀释时先变成蓝色，渐变成紫色，最后变为红色。最大吸收波长530nm。有刺激性。商品中常混杂有甲基藏红T。Colour Index Number 50240
  • 易溶于水成红色溶液，溶于乙醇成红色带黄红色荧光。对盐酸为蓝红色溶液，过多则呈紫色，大量过多则转为蓝色；对氢氧化钠则生成桲红色沉淀；硫酸则为绿色溶液，稀释时先变成蓝色，渐变成紫色，最后变为红色。最大吸收波长530nm。有刺激性。商品中常混杂有甲基藏红T。
  • 海关编码:32041300
  • 生产方法:以 2，5-二氨基甲苯、邻甲苯胺和苯胺为原料，首先将等摩尔的 2，5-二氨基甲苯与邻甲苯胺氧化，然后与苯胺缩合，通过精制结晶，转变成氯化物而得成品。
  • RTECS号:SG1623000

系列性分类

  1. Research Essentials 基础科研
  2. Core Bioreagents - Research Essentials
  3. Stains and Dyes - Core Bioreagents
  1. Life Science 生命科学
  2. Hematology and Histology 血液学和组织学
  3. Stains and Dyes - Hematology and Histology
  4. Certified Stains & Dyes - Stains and Dyes
  1. Life Science 生命科学
  2. Hematology and Histology 血液学和组织学
  3. Stains and Dyes - Hematology and Histology
  4. Stains & Dyes, A to Z - Stains and Dyes
  5. S - Stains & Dyes, A to Z
  1. Analytical & Chromatography 分析化学与色谱
  2. Analytical Reagents
  3. Titration - Analytical Reagents
  4. Indicators - Titration
  5. Redox Indicators - Indicators
  1. Life Science 生命科学
  2. Microbiology 微生物学
  3. Microscopy Reagents and Kits - Microbiology
  4. Staining Solutions - Microscopy Reagents and Kits
  5. Staining Solutions for Gram Staining - Staining Solutions
  1. Life Science 生命科学
  2. Microbiology 微生物学
  3. Microscopy Reagents and Kits - Microbiology
  4. Staining Solutions - Microscopy Reagents and Kits
  5. Staining Solutions for Spore Staining - Staining Solutions
  1. Analytical & Chromatography 分析化学与色谱
  2. Titration
  3. Indicators
  4. pH Indicators - Powders & Salts
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Hematology and Histology
  4. Stains and Dyes