Rhodamine B 罗丹明B

CAS 81-88-9 MFCD00011931

化学结构图

81-88-9
SMILES: CCN(CC)c1ccc2c(-c3ccccc3C(=O)O)c3ccc(=[N+](CC)CC)cc-3oc2c1.[Cl-]

化学属性

Mol. FormulaC28H31ClN2O3
Mol. Weight479.01
Density0.79
Melting Point210-211
TSCAYes
SolubilitySoluble in water (12 mg/ml), ethanol (70 mg/ml), acetone (slightly ), methyl cellosolve, and alcohol.
Stability对湿度敏感
Flash Point12°C
Appearance 亮绿色结晶性粉末。溶于水和乙醇呈蓝光红色 (带强荧光)，微溶于丙酮，极易溶于乙二醇乙醚。于浓硫酸中呈黄光棕色，带有较强的绿色荧光，稀释后呈猩红色，随后变为蓝光红色至橙色。其水溶液加入氢氧化钠呈玫瑰红色，加热后产生絮状沉淀。

别名和识别编码

Chemical NameRhodamine B
Synonym Aizen Rhodamine BHC Basazol Red 71P Basic Violet 10 C.I. Food Red 15 Calcozine Rhodamine BXP D and C Red No. 19 D&C Red 19 Diabasic Rhodamine B Edicol Supra Rose BS Eriosin Rhodamine B Ikada Rhodamine B Mitsui Rhodamine BX RHODAMINE Rheonine B Rhodam Rhodamin B Rhodamine B Rhodamine BXL Rhodamine O rhodamine B rhodamine B acetate rhodamine B chloride rhodamine B dihydride tetraethylrhodamine {} {hazard_com} ADC Rhodamine B {} {} {hazard_com} 9-o-Carboxyphenyl-6-diethylamino-3-ethylimino-3-isoxanthene, {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {uni_hamburg} Rhodamine BS
CAS Number81-88-9
EC Number201-383-9
Beilstein Registry Number4091619
MDL NumberMFCD00011931
PubChem Substance ID6694
Merck Number8183
Reaxys-RN4091619
Chemical Name Translation罗丹明B
Wiswesser Line NotationT C666 BO EYJ EUK2&2 IR BVQ& MN2&2 &Q &G
LabNetwork Molecule IDLN01310199
InChIInChI=1S/C28H30N2O3.ClH/c1-5-29(6-2)19-13-15-23-25(17-19)33-26-18-20(30(7-3)8-4)14-16-24(26)27(23)21-11-9-10-12-22(21)28(31)32;/h9-18H,5-8H2,1-4H3;1H
Canonical SMILESCCN(C1=CC2=[O+]C3=C(C=CC(N(CC)CC)=C3)C(C4=CC=CC=C4C(O)=O)=C2C=C1)CC.[Cl-]
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分类

  • {SNA} Analytical Reagents, Analytical/Chromatography, Derivatization Reagents, Derivatization Reagents TLC, Steroids, Terpenes, Lipids, Bile acids, TLC Reagents,
  • {SNA} Additional Standards, All alphabetical sorted, Analytical Standards, Chromatography, Colorant Standards, Colors, Food & Beverage Standards, Food and Cosmetic Component Standards, Standards for Personal Care and Cosmetic Pro
  • {SNA} Additional Standards, All alphabetical sorted, Analytical Standards, Chromatography, Colorant Standards, C
  • {SNA} Q-R, Stains & Dyes, A to Z, Stains and Dyes, 细胞生物学, 血液学和组织学

产品应用

  • A useful fluorochrome for histology, FRET and mitochondrial probe.

相关文献及参考

  • Short: II/3
  • Merck: 14,8183
  • Biological stain.
  • Merck: 14,8183 Beilstein: 19,V,8,669
  • Short: EINECS
  • Reagent for determination of Cd and Zn: Anal. Chem., 176, 285 (1960); also for various other metals.

安全信息

Signal word Danger
GHS Symbol
WGK Germany3
Safety Statements
  • S26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice 眼睛接触后，立即用大量水冲洗并征求医生意见；
  • S36/37/39 Wear suitable protective clothing, gloves and eye/face protection 穿戴适当的防护服、手套和眼睛/面保护；
Risk Statements
  • R11 Highly flammable 非常易燃
  • R22 Harmful if swallowed 吞咽有害
  • R36 Irritating to eyes 刺激眼睛
  • R40 Limited evidence of a carcinogenic effect 有限证据表明其致癌作用
  • R41 Risk of serious damage to eyes 有严重损伤眼睛的危险
  • R67 Vapours may cause drowsiness and dizziness 蒸汽可能导致嗜睡和昏厥
  • R68 Possible risk of irreversible effects 可能有不可挽回的作用的危险
Precautionary statements
  • P210 Keep away from heat/sparks/open flames/hot surfaces. — No smoking. 远离热源/火花/明火/热的表面。——禁止吸烟。
  • P260 Do not breathe dust/fume/gas/mist/vapours/spray. 不要吸入粉尘/烟/气体/烟雾/蒸汽/喷雾。
  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray. 避免吸入粉尘/烟/气体/烟雾/蒸汽/喷雾。
  • P264 Wash hands thoroughly after handling. 处理后要彻底洗净双手。
  • P264+P265
  • P270 Do not eat, drink or smoke when using this product. 使用本产品时不要吃东西，喝水或吸烟。
  • P271 Use only outdoors or in a well-ventilated area.? 只能在室外或通风良好的地方使用。
  • P272 Contaminated work clothing should not be allowed out of the workplace. 污染的工作服不得带出工作场所。
  • P273 Avoid release to the environment. 避免释放到环境中。
  • P280 Wear protective gloves/protective clothing/eye protection/face protection. 戴防护手套/防护服/眼睛的保护物/面部保护物。
  • P301+P312
  • P301+P312+P330
  • P301+P317
  • P302+P350
  • P302+P352+P333+P313+P362+P364
  • P304+P340
  • P305+P351+P338
  • P305+P351+P338+P310
  • P305+P354+P338
  • P310 Immediately call a POISON CENTER or doctor/physician. 立即呼救解毒中心或医生/医师。
  • P312 Call a POISON CENTER or doctor/physician if you feel unwell. 如果你感觉不适，呼叫解毒中心或医生/医师。
  • P317
  • P319
  • P330 Rinse mouth. 漱口
  • P333+P313
  • P403+P233
  • P405 Store locked up. 上锁保管。
  • P501 Dispose of contents/container to..… 处理内容物/容器.....
Hazard statements
  • H225 Highly flammable liquid and vapour 高度易燃液体和蒸气
  • H302 Harmful if swallowed 吞食有害
  • H318 Causes serious eye damage 严重伤害眼睛
  • H319 Causes serious eye irritation 严重刺激眼睛
  • H336 May cause drowsiness or dizziness 肯能导致嗜睡或头晕
  • H412 Harmfutoaquaticlifewithlonglastingeffects 对水生生物有害并具有长期影响。
Personal Protective Equipment Eyeshields, Faceshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter dust mask type N95 (US), Eyeshields, Gloves
RTECSBP3675000
Hazard Codes 6.1 Xn Xn,Xi,F
Packing GroupIII
UN Number 2811 UN 1219 3/PG 2
Storage condition 2-8°C, sealed storage, away from moisture and light {LY} 2-8°C, sealed storage, away from moisture and light Store at RT. {} {LY} 2-8°C, protect from light, stored under nitrogen 密封保存。 
TYPE OF TEST            : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE       : Subcutaneous
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 3870 mg/kg/68W-I
TOXIC EFFECTS :
   Tumorigenic - equivocal tumorigenic agent by RTECS criteria
   Tumorigenic - tumors at site of application
REFERENCE :
   GANNA2 Gann.  Japanese Journal of Cancer Research.  (Tokyo, Japan)  V.1-75,
   1907-84.  For publisher information, see JJCREP.  Volume(issue)/page/year:
   46,369,1955

TYPE OF TEST            : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE       : Subcutaneous
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 3600 mg/kg/68W-I
TOXIC EFFECTS :
   Tumorigenic - equivocal tumorigenic agent by RTECS criteria
   Blood - lymphoma, including Hodgkin's disease
   Tumorigenic - tumors at site of application
REFERENCE :
   GANNA2 Gann.  Japanese Journal of Cancer Research.  (Tokyo, Japan)  V.1-75,
   1907-84.  For publisher information, see JJCREP.  Volume(issue)/page/year:
   47,51,1956

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intraperitoneal
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 144 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   60LFAC "Recent Advances of Chemistry and Molecular Biology in Cancer
   Research, Insternational Symposium on Recent Advances of Chemistry and
   Molecular Biology in Cancer Research, Held in Beijing/China, July 23rd-26th,
   1991" Dai Qianhuan, Margaret-Ann Armour and Zheng Qingying eds. Science
   Press (Beijing) 1993  Volume(issue)/page/year: -,281,1993

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Oral
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 887 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   TOERD9 Toxicological European Research.  (S.P.E., 10, Ave. de Messine, 75008
   Paris, France) V.1-    1978-  Volume(issue)/page/year: 1,23,1978

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 89 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   TXAPA9 Toxicology and Applied Pharmacology.  (Academic Press, Inc., 1 E.
   First St., Duluth, MN 55802) V.1-    1959-  Volume(issue)/page/year:
   3,696,1961

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Subcutaneous
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 180 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   OKEHDW Osaka-furitsu Koshu Eisei Kenkyusho Kenkyu Hokoku, Koshu Eisei Hen.
   Research Reports of the Oaka Prefectural Institute of Public

TYPE OF TEST            : Sex chromosome loss and nondisjunction
ROUTE OF EXPOSURE       : Oral
TEST SYSTEM             : Insect - Drosophila melanogaster
DOSE/DURATION           : 1000 ppm
REFERENCE :
   AMNTA4 American Naturalist.  (Univ. of Chicago Press, Journals Div., POB
   37005, Chicago, IL 60637) V.1-    1867-  Volume(issue)/page/year:
   87,295,1953

TYPE OF TEST            : Phage inhibition capacity
TEST SYSTEM             : Bacteria - Escherichia coli
DOSE/DURATION           : 25 ug/well
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Netherlands) V.1-    1964-  Volume(issue)/page/year: 260,349,1991

TYPE OF TEST            : DNA damage
TEST SYSTEM             : Rodent - hamster Ovary
DOSE/DURATION           : 900 umol/plate
REFERENCE :
   AMNTA4 American Naturalist.  (Univ. of Chicago Press, Journals Div., POB
   37005, Chicago, IL 60637) V.1-    1867-  Volume(issue)/page/year:
   87,295,1953

TYPE OF TEST            : Cytogenetic analysis
TEST SYSTEM             : Rodent - hamster Lung
DOSE/DURATION           : 10 mg/L
REFERENCE :
   ATSUDG Archives of Toxicology, Supplement.  (Springer-Verlag New York, Inc.,
   Service Center, 44 Hartz Way, Secaucus, NJ 07094)  No.1-    1978-
   Volume(issue)/page/year: 4,41,1980

TYPE OF TEST            : Cytogenetic analysis
TEST SYSTEM             : Rodent - hamster Ovary
DOSE/DURATION           : 20 umol/L/5H (Continuous)
REFERENCE :
   ENMUDM Environmental Mutagenesis.  (New York, NY)  V.1-9, 1979-87.  For
   publisher information, see EMMUEG.  Volume(issue)/page/year: 1,27,1979

TYPE OF TEST            : Cytogenetic analysis
TEST SYSTEM             : Mammal - species unspecified Fibroblast
DOSE/DURATION           : 2 mg/L
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Netherlands) V.1-    1964-

TYPE OF TEST            : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE       : Intraperitoneal
SPECIES OBSERVED        : Rodent - mouse
DOSE                    : 60 mg/kg
SEX/DURATION            : female 7-10 day(s) after conception
TOXIC EFFECTS :
   Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death,
   e.g., stunted fetus)
REFERENCE :
   TJADAB Teratology, The International Journal of Abnormal Development. (Alan
   R. Liss, Inc., 41 E. 11th St., New York, NY 10003)  V.1-    1968-
   Volume(issue)/page/year: 40,143,1989

其他信息

  • 用途五:碱性玫瑰精 B 用于染蚕丝、腈纶、羊毛，日晒牢度可达 2-3 级，用于单宁媒染棉纤维，日晒牢度较差。也用于皮革、纸张、麦秆着色以及制备色淀颜料。
  • Alfa Aesar:玫瑰精B Rhodamine B(81-88-9)
  • 方法一:以间羟基二乙基苯胺 (m-diethylaminophenol) 和苯酐为原料，经缩合、碱熔、酸溶、结晶、干燥即得成品。。
  • 用途七:测定锑、铋、钴、金、锰、汞、钼、钽、铊、钨等试剂，吸附指示剂，生物染色剂。
  • 色指数:45170
  • 罗丹明 B价格(试剂级):更新日期 产品编号 产品名称 包装 价格 2011/08/23 71036314 玫瑰红B 25g 21元 2011/08/23 K8369001 罗丹明　B 25g 235元 2011/04/16 R0040 若丹明B Rhodamine B 250G 1190元
  • 下游产品:耐晒桃红色淀 --> 耐晒圆珠笔红 --> 醇溶耐晒火红B
  • 用途四:本品用于纸张、腈纶、丝绸、皮革、羽毛染色，并可用于食品和某些金属分析试剂
  • 用途三:用于造纸、油漆、腈纶、木制品染色
  • Acros Organics:罗丹明B Rhodamine B, pure(81-88-9)
  • 用途二:用作吸附指示剂和生物染色剂等
  • 溶于约70分水，10分醇，150分氯仿，水溶液为蓝红色，稀释后有强烈荧光，微溶于盐酸和氢化钠溶液。约260℃熔结，约280℃熔融。最大吸收波长451nm。半数致死量(小鼠，腹腔)150mg/kg。
  • Rhodamine B is a useful fluorochrome for histology due to its fluorescent properties in ultraviolet light. It ejects an electron onto TiO2, which is then scavenged by O2 forming the O2- radical anion. This anion ultimately becomes the OH radical. A pH-independent process degrades Rhodamine B in TiO2 dispersions containing no dodecylbenzenesulfonate. The compound has been incorporated in a FRET system based on lanthanide-doped nanoparticles for monitoring biological effects. Rhodamine B has also been used as a mitochondrial probe to study the energetic state of multi-drug resistant and sensitive cells where the mitochondrial Rhodamine B concentration was precisely determined with the loss of fluorescence in the presence of MTT Formazan ，a fluorescence quencher. In addition, Rhodamine B has been observed to significantly reduce the number of human lip fibroblast cells in culture. Used for staining Negri bodies in tissue.
  • Sigma Aldrich:81-88-9(sigmaaldrich)
  • 海关编码:32041300
  • MOL 文件:81-88-9.mol
  • 用途一:本品用于纸张、晴纶、丝绸、皮革、羽毛染色，并可用于食品和某些金属分析试剂
  • 上游原料:苯酐 --> 硫酸化蓖麻油 --> 3-羟基-N,N-二乙基苯胺 --> 黄糊精

系列性分类

  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Fluorescent Probes, Labels, Particles and Stains - Biochemicals and Reagents
  4. Detection - Fluorescent Probes, Labels, Particles and Stains
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Fluorescent Probes, Labels, Particles and Stains - Biochemicals and Reagents
  4. Detection - Fluorescent Probes, Labels, Particles and Stains
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Fluorescent Probes, Labels, Particles and Stains - Biochemicals and Reagents
  4. Fluorescent Indicators and Probes - Fluorescent Probes, Labels, Particles and Stains
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Luminescent Compounds/Detection - Biochemicals and Reagents
  4. Wavelength Index - Luminescent Compounds/Detection
  1. Analytical & Chromatography 分析化学与色谱
  2. Analytical Standards
  3. Alphabetic - Analytical Standards
  4. R - Alphabetic
  1. Life Science 生命科学
  2. Hematology and Histology 血液学和组织学
  3. Stains and Dyes - Hematology and Histology
  4. Stains & Dyes, A to Z - Stains and Dyes
  5. Q-R - Stains & Dyes, A to Z
  1. Analytical & Chromatography 分析化学与色谱
  2. Analytical Reagents
  3. Derivatization Reagents 衍生分析试剂
  4. Derivatization Reagents TLC - Derivatization Reagents
  5. TLC Visualization Reagents (alphabetic sort) - Derivatization Reagents TLC
  6. TLC Reagents, N-R - TLC Visualization Reagents (alphabetic sort)
  1. Analytical & Chromatography 分析化学与色谱
  2. Analytical Reagents
  3. Derivatization Reagents 衍生分析试剂
  4. Derivatization Reagents TLC - Derivatization Reagents
  5. TLC Reagents - Derivatization Reagents TLC
  1. Analytical & Chromatography 分析化学与色谱
  2. Ion Association
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Fluorescent Probes, Labels, Particles and Stains
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Luminescent Compounds/Detection
  4. Fluorescent Probes, Labels, Particles and Stains
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Luminescent Compounds/Detection
  4. Solvents, Buffers and Reagents for Luminescence
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Biochemicals and Reagents
  4. Stains and Dyes
  5. Natural Dyes