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分类

• {SNA} 4.2.x.x C-O bonds,
• 4.2.x.x C-O bonds, 4.x.x.x Lyases, Analytical and Industrial Enzymes, Application Index, Biochemicals and Reagents, Carbonic Anhydrase, Cell Signaling Enzymes, Enzyme Class Index, Enzymes, Inhibitors, and Substrates, Human Carbonic Anhydrase, Plasma & Blood Proteins, Plasma, Blood, and Related Proteins and Reagents, Proteins and Derivatives
• {SNA} 4.2.x.x C-O bonds, 4.x.x.x Lyases, Analytical and Industrial Enzymes, Application Index, Bovine Carbonic Anhydrase, Carbonic Anhydrase, Cell Signaling Enzymes, Enzyme Class Index, General Metabolic Enzymes, General Metabolic Enzymes A-H,
• {SNA} 4.2.x.x C-O bonds,

产品应用

• Carbonic anhydrase from bovine erythrocytes (BCA) has been used to study the effect of removal of enzyme-bound metal ion, Zn2+, on aggregation behavior of the enzyme. Removal of metal ion by a chelator such as EDTA enhances the propensity of the enzyme to adopt the molten-globule state. This state of the enzyme is found to bind to the chaperone-like α-crystallin and prevent aggregation. The enzyme from Aladdin has been immobilized to electrochemical transducers in order to obtain develop an analytical device for dissolved CO2 measurement. It has also been used for the thermodynamic analysis of conformational changes in BCA.

相关文献及参考

• Agnew, H.D., et al., Iterative in situ click chemistry creates antibody-like protein-capture agents. Angew. Chem. Int. Ed. Engl. 48 , 4944-8, (2009) 摘要
• Vernier, W., et al., Thioether Benzenesulfonamide Inhibitors Of Carbonic Anhydrases II And IV: Structure-based Drug Design, Synthesis, And Biological Evaluation. Bioorg. Med. Chem. 18 , 3307-19, (2010) 摘要
• Van Heeke, G., et al., Gene fusions with human carbonic anhydrase II for efficient expression and rapid single-step recovery of recombinant proteins: expression of the Escherichia coli F1-ATPase epsilon subunit Protein Expr. Purif. 4 , 265-74, (1993)
• Fisher, S., et al., Speeding up proton transfer in a fast enzyme: kinetic and crystallographic studies on the effect of hydrophobic amino acid substitutions in the active site of human carbonic anhydrase II Biochemistry 46 , 3803-13, (2007)
• Mikulski, R., and D. Silverman, Proton transfer in catalysis and the role of proton shuttles in carbonic anhydrase Biochim. Biophys. Acta 1804 , 422-6, (2010)
• Deutsch, H. Int. J. Biochem. 19 , 101, (1987)
• Holmes, R.S. Eur. J. Biochem. 78 , 511, (1977)
• Merck 14 ,1812

安全信息

其他信息

• Carbonic anyhdrase is useful in carboxy group transfers and reduction reactions.