{SNA} 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index, Enzymes for Diagnostic Kit Manufacturing, Enzymes, Inhibitors, and Substrates, General Metabolic Enzymes, Molecular Diagnostics, Nucleotide Metabolism, Protein & Nucleic Acid Isolation, 代谢组学, 细胞生物学
{SNA} 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Enzyme Class Index, Enzymes for Diagnostic Kit Manufacturing, General Metabolic Enzymes, Molecular Diagnostics, Nucleotide Metabolism, Protein & Nucleic Acid Isolation, 代谢组学,
{SNA} 3.1.x.x Acting on esters,
产品应用
Nuclease from Staphylococcus aureus has been used in a study to assess coagulase and heat-resistant strains found in animals. It has also been used in a study to investigate the expression characteristic of two genes in S. aureus that encode two thermostable nucleases.
安全信息
Personal Protective Equipment
Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany
3
Storage condition
储存温度 2-8°C
其他信息
Micrococcal nuclease catalyzes cleavage of both DNA and RNA to yield 3'-nucleotides. It exhibits exo- and endo-5'-phosphodiesterase activities. The enzyme catalyzes preferential endohydrolysis of the RNA and DNA at sites rich in adenylate or uridylate and deoxyadenylate or thymidylate. The enzyme has a molecular weight of 16,807 daltons and is calcium dependent. The pH optimum is 9.2 but varies depending upon the concentration of ionized calcium present.