Ethylenediaminetetraacetic acid 乙二胺四乙酸

CAS 60-00-4 MFCD00003541

化学结构图

60-00-4
SMILES: O=C(O)CN(CCN(CC(=O)O)CC(=O)O)CC(=O)O

化学属性

Mol. FormulaC10H16N2O8
Mol. Weight292.24
Melting Point250°C
TSCAYes
Refractive indexn20/D 1.363
Density0.860
Solubility25℃时在水中的溶解度为0.5g/L。不溶于冷水、醇和一般有机溶剂。溶于氢氧化钠、碳酸钠和氨溶液。
Boiling Point434.18 °C at 760 mmHg
Appearance crystalline 从水中结晶析出的本品为白色粉末。 25℃时在水中的溶解度为0.5g/L。不溶于冷水、醇和一般有机溶剂。溶于氢氧化钠、碳酸钠和氨溶液。
Flash Point325.2±31.5 °C

别名和识别编码

Chemical NameEthylenediaminetetraacetic acid
Synonym Ethylenediaminetetraacetic acid {LY} Ethylenediaminetetraacetic acid {} {LY} Ethylenediaminetetraacetic acid {} {} {LY} Ethylenediaminetetraacetic acid {} {} {} {LY} Ethylenediaminetetraacetic acid {} {} {} {} {LY} Ethylenediaminetetraacetic acid {} {} {} {} {} {LY} Ethylenediaminetetraacetic acid {} {} {} {} {} {} {LY} Ethylenediaminetetraacetic acid {} {} {} {} {} {} {} {LY} Ethylenediaminetetraacetic acid {} {} {} {} {} {} {} {} {LY} Ethylenediaminetetraacetic acid {} {} {} {} {} {} {} {} {} {LY} Ethylenediaminetetraacetic acid {} {} {} {} {} {} {} {} {} {} {LY} Ethylenediaminetetraacetic acid {} {} {} {} {} {} {} {} {} {} {} {LY} Ethylenediaminetetraacetic acid {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Ethylenediaminetetraacetic acid {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Ethylenediaminetetraacetic acid {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Ethylenediaminetetraacetic acid {} {} {} {} {} {} {} {} {} {} {} {} {}
CAS Number60-00-4
PubChem Substance ID6049
EC Number200-449-4
Beilstein Registry Number1716295
MDL NumberMFCD00003541
Merck Number3517
Chemical Name Translation乙二胺四乙酸
Reaxys-RN1716295
Wiswesser Line NotationQV1N1VQ2N1VQ1VQ
LabNetwork Molecule IDLN00196842
InChIInChI=1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)
Canonical SMILESO=C(O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
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分类

  • Biochemicals and Reagents, Calcium Chelators, Calcium Signaling Research Tools, Cell Biology, Cell Culture, Cell Culture Supplies, Cell Dissociation, Cell Signaling and Neuroscience, Chelators, Dissociation, Essential Chemicals, Ethylenediaminetetraacetic acid (EDTA) and EDTA Solutions, General Stem Cell Biology, Inorganic Salts, Solutions and Reagents, Intracellular Calcium Signaling, Labware, Non-Enzymatic Dissociation Reagents, Reagents and Supplements, Research Essentials, Scale-Up Cell Culture using Corning Roller Bottles, Stem Cell Biology, Stem Cell Isolation, Supplies for Scale-Up Cell Dissociation
  • {SNA} BioUltra,
  • {SNA} Antio

产品应用

  • 钙离子络合剂,洗涤剂,血液抗凝剂。生化研究中用作钙螯合剂,消除微量重金属导致的酶催化反应中的抑制作用。

相关文献及参考

  • Merck:14,3517
  • Widely-used complexing agent for many cations.
  • Merck:14,3517 Beilstein: 4,IV,2449

安全信息

GHS Symbol
WGK Germany2
Hazard statements
  • H319 Causes serious eye irritation 严重刺激眼睛
  • H303 May be harmfully swallowed 吞食可能有害
Personal Protective Equipment Eyeshields, Gloves dust mask type N95 (US), Eyeshields, Gloves
Hazard Codes Xi
RTECSAH4025000
Precautionary statements
  • P201 Obtain special instructions before use. 使用前获取专门指示。
  • P202 Do not handle until all safety precautions have been read and understood. 已阅读并理解所有的安全预防措施之前,切勿操作。
  • P260 Do not breathe dust/fume/gas/mist/vapours/spray. 不要吸入粉尘/烟/气体/烟雾/蒸汽/喷雾。
  • P264 Wash hands thoroughly after handling. 处理后要彻底洗净双手。
  • P270 Do not eat, drink or smoke when using this product. 使用本产品时不要吃东西,喝水或吸烟。
  • P280 Wear protective gloves/protective clothing/eye protection/face protection. 戴防护手套/防护服/眼睛的保护物/面部保护物。
  • P281 Use personal protective equipment as required. 使用所需的个人防护装备。
  • P305+P351+P338
  • P305+P351+P338+P337+P313
  • P308+P313
  • P391 Collect spillage. Hazardous to the aquatic environment 收集对水环境有危害的泄漏物。
  • P405 Store locked up. 上锁保管。
  • P501 Dispose of contents/container to..… 处理内容物/容器.....
Signal word Warning
Safety Statements
  • S26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice 眼睛接触后,立即用大量水冲洗并征求医生意见;
  • S36 Wear suitable protective clothing 穿戴适当的防护服;
  • S37/39 Wear suitable gloves and eye/face protection 穿戴适当的手套和眼睛/面保护;
  • S61 Avoid release to the environment. Refer to special instructions/safety data sheet 避免释放到环境中,参考特别指示/安全收据说明书;
Risk Statements
  • R36 Irritating to eyes 刺激眼睛
  • R52/53
Packing GroupIII
UN Number 3077
Storage condition RT, protect from light, stored under nitrogen {LY} RT, protect from light, stored under nitrogen {} {LY} RT, protect from light, stored under nitrogen {} {} {LY} RT, protect from light, stored under nitrogen {} {} {} {LY} RT, protect from light, stored under nitrogen {} {} {} {} {LY} RT, protect from light, stored under nitrogen {} {} {} {} {} {LY} RT, protect from light, stored under nitrogen {} {} {} {} {} {} {LY} RT, protect from light, stored under nitrogen {} {} {} {} {} {} {} {LY} RT, protect from light, stored under nitrogen {} {} {} {} {} {} {} {} {LY} RT, protect from light, stored under nitrogen {} {} {} {} {} {} {} {} {} {LY} RT, protect from light, stored under nitrogen {} {} {} {} {} {} {} {} {} {} {LY} RT, protect from light, stored under nitrogen {} {} {} {} {} {} {} {} {} {} {} {LY} RT, protect from light, stored under nitrogen {} {} {} {} {} {} {} {} {} {} {} {} {LY} RT, protect from light, stored under nitrogen {} {} {} {} {} {} {} {} {} {} {} {}
Hazard Class9
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Oral
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 30 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   FCTOD7 Food and Chemical Toxicology.  (Pergamon Press Inc., Maxwell House,
   Fairview Park, Elmsford, NY 10523)  V.20-    1982- Volume(issue)/page/year:
   29,845,1991

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 28500 ug/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   JJPAAZ Japanese Journal of Pharmacology.  (Japanese Pharmacological Soc.,
   c/o Dept. of Pharmacology, Faculty of Medicine, Kyoto Univ., Sakyo-ku, Kyoto
   606, Japan)  V.1-    1951-  Volume(issue)/page/year: 63,187,1993

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intraperitoneal
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 250 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   NTIS** National Technical Information Service.  (Springfield, VA 22161)
   Formerly U.S. Clearinghouse for Scientific & Technical Information.
   Volume(issue)/page/year: AD691-490

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intraperitoneal
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 397 mg/kg
TOXIC EFFECTS :
   Behavioral - convulsions or effect on seizure threshold
REFERENCE :
   AHRTAN Arhiv za Higijenu Rada i Toksikologiju.  Archives of Industrial
   Hygiene and Toxicology. (Jugoslovenska Knjiga, POB 36, YU-11001 Belgrade,
   Yugoslavia)  V.7-    1956-  Volume(issue)/page/year: 13,295,1962

TYPE OF TEST            : Mutation in mammalian somatic cells
TEST SYSTEM             : Rodent - mouse Lymphocyte
DOSE/DURATION           : 25200 umol/L
REFERENCE :
   MUTAEX Mutagenesis. (Oxford Univ. Press, Pinkhill House, Southfield Road,
   Eynsham, Oxford OX8 1JJ, UK) V.1- 1986-  Volume(issue)/page/year: 3,193,1988

TYPE OF TEST            : Unscheduled DNA synthesis
TEST SYSTEM             : Rodent - hamster Embryo
DOSE/DURATION           : 100 umol/L
REFERENCE :
   SHIGAZ Shigaku.  Ondotology.  (Nippon Shika Daigaku Shigakkai, 1-9-20
   Fujimi, Chiyodaku, Tokyo 102, Japan)  V.38-    1949-
   Volume(issue)/page/year: 74,1365,1987

TYPE OF TEST            : Cytogenetic analysis
ROUTE OF EXPOSURE       : Intraperitoneal
TEST SYSTEM             : Rodent - mouse
DOSE/DURATION           : 50 mmol/L
REFERENCE :
   CISCB7 CIS, Chromosome Information Service.  (Maruzen Co. Ltd., POB 5050,
   Tokyo International, Tokyo 100-31, Japan)  No.1-    1961-
   Volume(issue)/page/year: 20,28,1976

TYPE OF TEST            : DNA damage
TEST SYSTEM             : Rodent - mouse Lymphocyte
DOSE/DURATION           : 40500 umol/L
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Scien

TYPE OF TEST            : Micronucleus test
ROUTE OF EXPOSURE       : Oral
TEST SYSTEM             : Rodent - mouse
DOSE/DURATION           : 15 mg/kg
REFERENCE :
   FCTOD7 Food and Chemical Toxicology.  (Pergamon Press Inc., Maxwell House,
   Fairview Park, Elmsford, NY 10523)  V.20-    1982- Volume(issue)/page/year:
   29,845,1991

TYPE OF TEST            : DNA inhibition
TEST SYSTEM             : Rodent - rabbit Kidney
DOSE/DURATION           : 250 umol/L
REFERENCE :
   ECREAL Experimental Cell Research.  (Academic Press, Inc., 1 E. First St.,
   Duluth, MN 55802) V.10-    1950-  Volume(issue)/page/year: 36,92,1964

TYPE OF TEST            : DNA inhibition
TEST SYSTEM

TYPE OF TEST            : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE       : Oral
SPECIES OBSERVED        : Rodent - rat
DOSE                    : 7632 mg/kg
SEX/DURATION            : female 7-14 day(s) after conception
TOXIC EFFECTS :
   Reproductive - Specific Developmental Abnormalities - cardiovascular
   (circulatory) system
   Reproductive - Specific Developmental Abnormalities - respiratory system
   Reproductive - Specific Developmental Abnormalities - urogenital system
REFERENCE :
   TXAPA9 Toxicology and Applied Pharmacology.  (Academic Press, Inc., 1 E.
   First St., Duluth, MN 5

TYPE OF TEST            : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE       : Oral
SPECIES OBSERVED        : Rodent - rat
DOSE                    : 7632 mg/kg
SEX/DURATION            : female 7-14 day(s) after conception
TOXIC EFFECTS :
   Reproductive - Specific Developmental Abnormalities - eye/ear
   Reproductive - Specific Developmental Abnormalities - craniofacial
   (including nose and tongue)
   Reproductive - Specific Developmental Abnormalities - musculoskeletal system
REFERENCE :
   TXAPA9 Toxicology and Applied Pharmacology.  (Academic Press, Inc., 1 E.
   First St., Duluth, MN 55802) V.1-    1959-  Volume(issue)/page/year:
   40,299,1977

TYPE OF TEST            : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE       : Oral
SPECIES OBSERVED        : Rodent - rat
DOSE                    : 7632 mg/kg
SEX/DURATION            : female 7-14 day(s) after conception
TOXIC EFFECTS :
   Reproductive - Fertility - post-implantation mortality (e.g. dead and/or
   resorbed implants per total number of implants)
   Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death,
   e.g., stunted fetus)
REFERENCE :
   TXAPA9 Toxicology and Applied Pharmacology.  (Academic Press, Inc., 1 E.
   First St., Duluth, MN 55802) V.1-    1959-  Volume(issue)/page/year:
   40,299,1977

其他信息

  • 含量分析:精确称取试样约5g,移入一250ml容量瓶中,加水溶解并定容后混匀,以此作为试样液。精确称取已知纯度的试剂级碳酸钙约200mg,置于400ml烧杯中,
  • 用途八:络合剂,钙、
  • Alfa Aesar:乙二胺四乙酸二钠盐二水合物,ACS,99.0-101.0% Ethylenediaminetetraacetic acid disodium salt dihydrate, ACS, 99.0-101.0%(6381-92-6)
  • {Chem
  • 上游原料:氢氧化钠 --> 盐酸 --> 乙二胺 -->
  • MOL 文件:6381-92-6.mol
  • 参考质量标准一:FAO/WHO,1978 含量/
  • 乙二胺四乙酸二钠盐价格(试剂级):更新日期 产品编号 产品名称 包装 价格 2011/08/23 53101561 乙二胺四乙酸二钠盐;氨羧络合剂III;螯合剂III;络合酮II 500ml 80元 2011/08/23 53101562 乙二胺四乙酸二钠盐;氨羧络合剂III;螯合剂III;络合酮II 500ml 80元 201
  • 外观性质:白色晶体。
  • 用途六:乙二胺四乙酸的盐类中,二钠盐最为重要,是一种重要络合剂,用于络合金属离子和分离金属,也可用于洗涤剂、液体肥皂、洗发剂、农业化学喷雾剂、彩色感光材料冲洗加工漂白定影液、净水剂、pH调节剂、阴凝剂等。在丁苯橡胶聚合用氧化还原引发系统中,EDTA二钠作为活性剂的组成部分,主要用于络合亚铁离子,控制聚合反应速度。本品低毒,大鼠口服LD50为2000毫克/公斤。
  • 用途七:络合剂,钙、镁及其他金属试剂,金属掩蔽剂。乙二胺四乙酸二钠是一种重要络合剂,用于络合金属离子和分离金属,也可用于洗涤剂,液体肥皂,洗发剂,农业化学喷雾剂,彩色感光材料冲洗,加工漂白定影剂,净水剂,PH调节剂,阻凝剂等,还可用作分析用的基准试剂,隐蔽剂等。校准仪器和装置;评价方法;工作标准;质量保证/质量控制;其他。
  • 上游原料:氢氧化钠 --> 盐酸 --> 乙二胺 --> 氯乙酸 --> 浓硫酸 --> 乙二胺四乙酸 --
  • 毒性:ADI 0~0.2mg/kg(FAO/WHO,2001)。 LD 50 2g/kg(大鼠,经口)。 可安全用于食品(FDA,§172.135.2000)。
  • {Chemi
  • 方法二:氰化钠法 按一定的配比将氰化钠与甲醛的混合物缓缓加入乙二胺水溶液中,在85℃、减压下通入空气以除去氨气。反应完毕后,用浓硫酸调Ph值至4. 5,再经脱色、过滤、浓缩、结晶分离、干燥得成品。氯乙酸法 先将100kg氯乙酸、100kg冰和135kg 30%的NaOH溶液混合,在搅拌下加入18kg 83%~84%的乙二胺,在15℃下保温1h。分批缓慢加入30%的NaOH溶液至反应物显碱性为止,并在室温下保持1
  • 使用限量:GB 2760—96:酱菜、罐头0.25g/
  • 防护措施:本品低毒。对大鼠经口LD50为2000mg/kg。
  • 含量分析:精确称取试样约5g,移入一250ml容量瓶中,加水溶解并定容后混匀,以此作为试样液。精确称取已知纯度的试剂级碳酸钙约2
  • 能溶于氢氧化钠、碳酸钠及氨溶液中,能溶于160分沸水,微溶于冷水,不溶于醇及一般有机溶剂。
  • 下游产品:百草枯 --> 乙二胺四乙酸四钠 --> 稳定剂 --> 硫酸博莱霉素 --> 废纸脱墨剂 --> 2,4,7-三氨基-6-苯基蝶啶 --> 高效煮练剂 KR-75 --> 特效去油灵 --> 双鲸CAO氧化胺 --> JC-832铜清洗剂
  • 所属类别一:食品添加剂: 螯合剂
  • 能溶于氢氧化钠、碳酸钠及氨溶液中,能溶于164分沸水,微溶于冷水,不溶于醇及一般有机溶剂。
  • F:3
  • 方法三:由
  • 能溶于氢氧化钠、碳酸钠及氨溶液中,能溶于161分沸水,微溶于冷水,不溶于醇及一般有机溶剂。
  • 用途二:用于氧羧络合剂,还用于制药工业
  • {Chemical

系列性分类